High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules
نویسندگان
چکیده
منابع مشابه
High-throughput single-molecule fluorescence spectroscopy using parallel detection.
Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept of this technique is to excite and collect light from a very small volume (typically femtoliter) and work in a concentration regime resulting in rare burst-like events corresponding to the transit of a single-molecule. Those even...
متن کاملMultispot single-molecule FRET: High-throughput analysis of freely diffusing molecules
We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be properly corrected and result in measured sample characteristics consistent with those obtained w...
متن کاملA microfluidic chip-compatible bioassay based on single-molecule detection with high sensitivity and multiplexing.
Many applications in pharmaceutical development, clinical diagnostics, and biological research demand rapid detection of multiple analytes (multiplexed detection) in a minimal volume. This need has led to the development of several novel array-based sensors. The most successful of these so far have been suspension arrays based on polystyrene beads. However, the 5 microm beads used for these ass...
متن کاملAn RF-powered micro-reactor for the detection of astrobiological target molecules on planetary bodies.
We describe a sample-processing micro-reactor that utilizes 60 GHz RF radiation with approximately 730 mW of output power. The instrument design and performance characterization are described and then illustrated with modeling and experimental studies. The micro-reactor's efficiency on affecting hydrolysis of chemical bonds similar to those within large complex molecules was demonstrated: a dis...
متن کاملHigh-throughput single-molecule studies of protein-DNA interactions.
Fluorescence and force-based single-molecule studies of protein-nucleic acid interactions continue to shed critical insights into many aspects of DNA and RNA processing. As single-molecule assays are inherently low-throughput, obtaining statistically relevant datasets remains a major challenge. Additionally, most fluorescence-based single-molecule particle-tracking assays are limited to observi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2018
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c8lc00535d